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BYF Genomic DNA Kit (E1085) – For Bacteria/Yeast/Fungi

Fiyat Sorunuz

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Rapid and reliable genomic DNA purification kit for Gram(-) and Gram(+) bacteria, yeast, and fungal cells. No phenol-chloroform required, delivers results in less than 25 minutes.

  • Genomic DNA isolation from Gram(-) and Gram(+) bacteria, yeast, and fungal cells
  • Silica membrane technology—phenol-chloroform-free
  • Protocol duration under 25 minutes
  • PCR, qPCR, sequencing, and enzymatic reaction-compatible purity
  • No alcohol precipitation or resin-based extraction required

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The BYF Genomic DNA Kit is designed for researchers performing genomic DNA isolation from bacterial, yeast, and fungal cells in microbiology and molecular biology laboratories. Using silica-based membrane technology, high-purity DNA is obtained without the need for hazardous phenol-chloroform extraction or time-consuming alcohol precipitation steps.

The kit includes protocols optimized for disrupting both Gram-negative and Gram-positive bacterial cell walls, ensuring consistent yields across different microorganism types. The standard protocol is completed in less than 25 minutes, representing significant time savings for laboratories processing large sample numbers.

The isolated DNA is suitable for direct use in PCR, qPCR, sequencing, and various enzymatic reactions without requiring additional purification steps. The column/membrane-based system offers reduced chemical hazard exposure and a simplified workflow compared to conventional methods.

Technical Specifications

Application Genomic DNA purification
Sample Type Bacteria (Gram+/Gram-), yeast, fungi
Technology Silica-based membrane
Protocol Duration Less than 25 minutes
Downstream Compatibility PCR, qPCR, sequencing, enzymatic reactions

Applications

  • Microbial genomic DNA isolation
  • Sample preparation prior to PCR and qPCR
  • DNA extraction for bacterial/yeast/fungal sequencing studies

Frequently Asked Questions

Which organism types can this kit isolate DNA from?
The kit is suitable for genomic DNA isolation from Gram-negative and Gram-positive bacteria, yeast, and fungal cells.
Is phenol-chloroform extraction required?
No. The kit uses silica membrane technology to eliminate this hazardous and time-consuming step.
What applications can the purified DNA be used for?
The purified DNA can be used directly in PCR, qPCR, sequencing, and enzymatic reactions.