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ClearBand DNA Marker 100bp-Green

Fiyat Sorunuz

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Ready-to-load molecular weight marker for precise DNA fragment size determination in agarose gel electrophoresis

12 distinct bands spanning 100 bp – 3 kb range
Enhanced intensity at 500 bp and 1,500 bp reference bands
Pre-loaded and ready-to-use format
Suitable for PCR products and restriction enzyme digests
Approximate molecular mass data for quantitative estimation

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Daha fazla detay

ClearBand DNA Marker 100bp-Green is a pre-loaded molecular weight standard marker designed for accurate determination of DNA fragment sizes in gel electrophoresis. Comprising PCR products and double-stranded DNA digested with appropriate restriction enzymes, it delivers 12 discrete bands ranging from 100 bp to 3 kb in agarose gel electrophoresis for molecular weight standardization. The marker bands at 500 and 1,500 bp feature enhanced intensity to serve as rapid reference points during analysis. Approximate molecular mass values are provided for each DNA band, enabling accurate estimation of DNA mass in comparably dense DNA samples relative to the marker ladder (based on 5 µL loading volume).

Technical Specifications

Marker Range	100 bp – 3 kb
Number of Bands	12
Enhanced Intensity Bands	500 bp, 1,500 bp
Recommended Loading Volume	5 µL
Applications	DNA fragment size determination, molecular weight standardization

Applications

PCR product size analysis
Restriction enzyme digest characterization
Agarose gel electrophoresis standardization
DNA fragment mass estimation
Molecular weight reference in gel-based DNA analysis

Frequently Asked Questions

What is the maximum DNA fragment size this marker can resolve?

The marker resolves DNA fragments up to 3 kb (3,000 bp).

Why are the 500 bp and 1,500 bp bands enhanced in intensity?

These bands serve as rapid reference points and are intentionally enhanced to facilitate quick visual orientation and band recognition during gel analysis.

How should this marker be used for DNA mass estimation?

The approximate molecular mass data provided for each band allows estimation of DNA mass in your samples by visual comparison with the marker ladder, assuming a 5 µL loading volume.

Is this marker suitable for both PCR products and enzymatically digested DNA?

Yes, the marker is formulated from PCR products and restriction enzyme-digested double-stranded DNA, making it suitable for reference in both applications.