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NutriCulture Cell Viability Detection Kit-8 (CVDK-8)

Fiyat Sorunuz

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High-sensitivity WST-8 based cell viability assay for culture applications

Water-soluble WST-8 tetrazolium salt formulation
Single-bottle ready-to-use solution format
Superior sensitivity compared to MTT, XTT, MTS, and WST-1
Suitable for cell proliferation assays
Suitable for cytotoxicity assessment
Electron mediator-enhanced formazan production

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Daha fazla detay

NutriCulture Cell Viability Detection Kit-8 (CVDK-8) utilizes water-soluble tetrazolium salt to enable highly convenient analytical procedures. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt], upon reduction in the presence of an electron mediator, produces a water-soluble formazan dye. NutriCulture CVDK-8 is provided as a single-bottle solution; pre-mixing of components is not required. NutriCulture CVDK-8 permits sensitive colorimetric assays for quantitation of viable cell numbers in cell proliferation and cytotoxicity experiments. WST-8 is reduced by dehydrogenases in cells to produce an orange-colored product (formazan) that is soluble in cell culture medium. The amount of formazan dye produced by dehydrogenases in cells is directly proportional to the number of viable cells. Detection sensitivity achieved using NutriCulture CVDK-8 is higher than those obtained using other tetrazolium salts such as MTT, XTT, MTS, or WST-1.

Technical Specifications

Active Reagent	WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt]
Product Format	Single-bottle solution
Solubility	Water-soluble formazan product
Detection Method	Colorimetric
Pre-treatment Required	None; ready-to-use

Applications

Cell viability assessment in mammalian cell culture
Cell proliferation assays
Cytotoxicity screening
Compound toxicity testing
Cell viability in primary cultures
Comparative sensitivity applications requiring MTT/XTT/MTS alternatives

Frequently Asked Questions

Is pre-mixing of kit components necessary?

No, NutriCulture CVDK-8 is provided as a single-bottle solution requiring no component pre-mixing.

What is the mechanism of color development?

WST-8 tetrazolium salt is reduced by cellular dehydrogenases to produce an orange-colored, water-soluble formazan dye that remains soluble in culture medium.

How does sensitivity compare to other tetrazolium assays?

NutriCulture CVDK-8 demonstrates higher detection sensitivity than MTT, XTT, MTS, and WST-1 based assays.

Is formazan product removal necessary?

No; the formazan product is water-soluble and does not require medium removal before measurement.

What is the relationship between formazan production and cell viability?

Formazan dye quantity produced by cellular dehydrogenases is directly proportional to viable cell number.