SENSISpec ELISA Macadamia Fıstığı Alerjen 96 Kuyucuk

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SENSISpec ELISA Macadamia Allergen 96 Wells

Fiyat Sorunuz

High-sensitivity sandwich ELISA kit for macadamia allergen detection and quantification in food matrices

  • Highly sensitive 96-well sandwich enzyme immunoassay
  • Detection limit (LOD): 0.1 ppm
  • Quantitation range: 1–40 ppm
  • Incubation protocol: 60 minutes total (20/20/20 min)
  • Minimal cross-reactivity: Hazelnut 0.0002%, Walnut 0.001%
  • 13-month shelf life at 2–8°C
  • HRP + TMB colorimetric readout at 450 nm
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2-3 Gün

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1-3 Gün

Ücretsiz Kargo

  • Garanti 2 yıl

Daha fazla detay

  • 14 Günlük ücretsiz iade

Daha fazla detay

The SENSISpec ELISA Macadamia Allergen is a robust detection system for quantifying macadamia residues in food products. The protein fraction in macadamia nuts (~7.9%) is relatively low compared to other nuts, yet certain protein components exhibit allergenic properties. While macadamia allergies remain uncommon, severe cases can trigger anaphylactic reactions at trace levels. Cross-contamination during manufacturing is frequent, necessitating sensitive analytical methods to detect and quantify macadamia residues. This kit delivers precise determination of macadamia allergen residues across complex food matrices including baked goods, cereals, ice cream, and chocolate.

Technical Specifications

SKU HU0030013
Test Principle Sandwich enzyme immunoassay (ELISA)
Format 96-well microplate
Total Incubation Time 60 minutes (20/20/20 min sequential incubations)
Standard Range 0, 1, 4, 10, 40 pg/min
Detection Wavelength 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) 0.1 ppm
Quantitation Range 1–40 ppm
Cross-Reactivity Hazelnut 0.0002%, Walnut 0.001%
Shelf Life 13 months
Storage Conditions 2–8°C

Applications

  • Bakery products and cookies
  • Cereal formulations
  • Ice cream and frozen confections
  • Chocolate and chocolate-based products
  • Quality assurance and allergen control programs
  • Cross-contamination monitoring in manufacturing

Frequently Asked Questions

Why is sensitive macadamia allergen detection necessary?
Although macadamia allergies are relatively uncommon, they can be severe and trigger anaphylactic reactions at trace levels. Additionally, cross-contamination during food production frequently introduces macadamia residues, making sensitive detection essential for food safety labeling and allergen control.
What is the analytical range of this ELISA kit?
The assay provides reliable quantitation from 1 to 40 ppm, with a limit of detection at 0.1 ppm. This range covers typical allergen residue levels encountered in processed food matrices.
Does this kit cross-react with other nuts?
Cross-reactivity is minimal: hazelnut at 0.0002% and walnut at 0.001%. This specificity ensures accurate macadamia quantitation without false-positive signals from botanical relatives.
What detection method is employed?
The assay uses horseradish peroxidase (HRP) enzyme linked to detection antibodies with TMB substrate, read colorimetrically at 450 nm on a standard microplate reader.

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