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Ovalbumin ELISA Kit SENSISpec 96 Wells Allergen Test

Fiyat Sorunuz

High-sensitivity ELISA kit for ovalbumin detection and quantification, optimized for wine analysis and allergen testing applications.

  • Sandwich enzyme immunoassay format
  • Limit of Detection: 4 ppb
  • Quantification range: 25–500 ppb
  • Optimized for wine and beverage analysis
  • Pre-calibrated with reference standards
  • 60-minute total incubation protocol

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The SENSISpec Ovalbumin ELISA Kit represents a highly sensitive detection system for ovalbumin and is particularly well-suited for quantifying ovalbumin residues in wine. Chicken egg (Gallus gallus) is protein-rich and represents an important food source for humans. While egg yolk proteins have only minor allergenic properties, many proteins in egg white are known allergens. Along with lysozyme, ovomucoid, ovotransferrin, and livetin, ovalbumin represents a major fraction of egg white allergens. Egg powder and purified ovalbumin are frequently used as clarifying agents in winemaking. For allergic individuals, ovalbumin consumption represents a critical concern. Even minute quantities of allergen can trigger allergic reactions that may lead to anaphylactic shock in severe cases. This sandwich enzyme immunoassay kit enables reliable detection and quantification of ovalbumin at clinically and analytically significant levels.

Technical Specifications

Stock Code HU0030017
Test Principle Sandwich enzyme immunoassay
Format 48-well Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Reference Standards 0; 25, 100; 250; 500 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 4 ppb
Quantification Range 25 – 500 ppb
Storage Temperature 2–8°C

Applications

  • Wine and beverage allergen screening
  • Ovalbumin residue quantification in clarified wines
  • Food manufacturing quality control
  • Allergen compliance testing and certification
  • Clinical diagnostic applications for egg allergy assessment
  • Regulatory compliance and food safety monitoring

Frequently Asked Questions

What is the primary application of the SENSISpec Ovalbumin ELISA Kit?
This kit is designed for sensitive detection and quantification of ovalbumin residues, particularly in wine samples treated with egg white as a clarifying agent. It is also applicable to food products and clinical diagnostics related to egg allergy assessment.
What detection method does this kit employ?
The kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) format with HRP/TMB (horseradish peroxidase/tetramethylbenzidine) detection at 450 nm wavelength.
What is the limit of detection for this assay?
The limit of detection (LOD) is 4 ppb (parts per billion), enabling detection of trace ovalbumin levels relevant to allergen-sensitive populations.
What is the working quantification range?
The linear quantification range is 25–500 ppb, with reference standards provided at 0, 25, 100, 250, and 500 ppb levels.
How should the kit be stored?
Store all kit components at 2–8°C (refrigerated conditions). Maintain appropriate storage temperature to preserve antibody activity and assay performance.
What is the total assay duration?
Total incubation time is 60 minutes, distributed as 20 minutes for each of three sequential incubation steps, allowing for efficient batch processing.