SENSISpec Walnut ELISA Kit (96-Well) – HU0030024

The SENSISpec Walnut ELISA Kit is a sandwich ELISA assay designed for quantitative detection of walnut residue in processed foods (cookies, ice cream, chocolate, cereal products, etc.). Manufactured by Gold Standard Diagnostics, the kit is routinely used in food safety and allergen control laboratory workflows.

The assay is completed with a short incubation period of 60 minutes total (20/20/20 minutes) and is read at 450 nm using an HRP+TMB system. With a detection limit of 0.35 ppm and a quantitation range of 2–60 ppm, the kit reliably detects even low-level walnut contamination. The kit includes standards at 0, 2, 6, 20, and 60 ppm, enabling direct calibration curve establishment.

Cross-reactivity data indicate that the assay exhibits measurable cross-reactivity with certain tree nuts (hazelnut 0.022%, chestnut 0.11%, pecan 0.80%), while demonstrating negligible reactivity (<0.003%) with soy, pine nut, peanut, sesame, and Brazil nut. These data should be considered when interpreting results.

With a 13-month shelf life and storage at 2–8°C, the kit is suitable for storage under standard laboratory refrigeration conditions.

Technical Specifications

Applications

• Screening for hidden walnut residue in processed foods (cookies, chocolate, ice cream, cereal products)
• In-line quality control for allergen labeling compliance during production
• Routine allergen analysis in food safety laboratories