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Sesame ELISA Kit SENSISpec 96 Wells – Allergen Test

Fiyat Sorunuz

High-sensitivity detection system for sesame residue quantification in food matrices

  • Sandwich enzyme immunological test principle
  • Quantification range: 2-30 ppm
  • Incubation time: 60 minutes (20/20/20 minutes)
  • Detection at 450 nm (HRP + TMB)
  • Minimal cross-reactivity with food matrices
  • 18-month shelf life at 2-8°C storage

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The SENSISpec Sesame ELISA kit represents a highly sensitive detection system for sesame residues, particularly suitable for quantification in flour products, soups, sausages, sauces, and ice cream. Sesame seeds contain a protein fraction of approximately 16-32%, with several of these proteins possessing allergenic properties including albumins (Ses i 1 and Ses i 2) and globulins (Ses i 3). Due to the widespread application of sesame in food preparation, trace amounts can be present in many food products. For individuals with sesame allergy, hidden sesame allergens in food represent a critical concern. Even minimal quantities of sesame can trigger allergic reactions that may result in anaphylactic shock in severe cases. Therefore, individuals with sesame allergy must strictly avoid consumption of foods containing sesame.

Technical Specifications

SKU HU0030022
Test Principle Sandwich enzyme immunoassay
Format 48-well microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 2; 5; 15; 30 ppm
Detection Wavelength 450 nm (HRP + TMB)
Quantitation Range 2 – 30 ppm
Cross-Reactivity Soup 0.2 ppm, Ice cream 0.2 ppm, Sausage 0.2 ppm, Salad dressing 0.2 ppm, Cracker 0.2 ppm, Oat 0.0003%, Bean 0.0003%
Shelf Life 18 months
Storage Conditions 2–8°C

Applications

  • Quantification of sesame residues in flour-based products
  • Sesame allergen detection in soup preparations
  • Allergen testing in meat products and sausages
  • Quality control in sauce and condiment manufacturing
  • Sesame contamination assessment in ice cream production
  • Regulatory compliance testing for allergen labeling
  • Cross-contamination monitoring in food processing facilities

Frequently Asked Questions

What is the sensitivity range of this ELISA kit?
The SENSISpec Sesame ELISA kit provides quantitation in the range of 2-30 ppm, with standards at 0, 2, 5, 15, and 30 ppm for accurate dose-response curves.
How long does the complete test procedure require?
Total incubation time is 60 minutes with the protocol structured as 20/20/20 minutes for sequential incubation steps.
What is the shelf life and storage requirement?
The kit has an 18-month shelf life when stored at 2–8°C. Proper temperature maintenance is critical for optimal performance.
Does this kit show cross-reactivity with other allergens?
Cross-reactivity testing shows minimal interference with common food matrices. Residual levels include 0.2 ppm in soup, ice cream, sausage, and dressing, and 0.0003% for oat and bean.
What detection method is employed?
The kit utilizes HRP (horseradish peroxidase) enzyme with TMB substrate, with optical density measured at 450 nm.
What protein allergens are present in sesame?
Sesame seeds contain 16-32% protein fraction. Major allergenic proteins include Ses i 1 and Ses i 2 (albumins) and Ses i 3 (globulin).