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Pistachio ELISA Kit SENSISpec 96 Wells

Fiyat Sorunuz

High-sensitivity sandwich ELISA assay for rapid pistachio allergen detection and quantification in food matrices and environmental surfaces

  • Sandwich enzyme immunological test principle for superior specificity
  • 96-well microplate format for high-throughput screening
  • Low limit of detection (LOD): 0.13 ppm
  • Rapid incubation protocol: 60 minutes total (20/20/20 minute intervals)
  • Quantitation range: 1–40 ppm for accurate allergen profiling
  • Extended shelf life: 13 months at 2–8°C
  • Cross-reactivity data available for related tree nuts

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The SENSISpec Pistachio ELISA is a sensitive and user-friendly detection system designed to identify pistachio allergen presence in food products and surfaces. Engineered for food safety and allergen management laboratories and manufacturers, this kit utilizes ELISA (Enzyme-Linked Immunosorbent Assay) technology to deliver high specificity and sensitivity. The 96-well microplate format accommodates small to mid-sized laboratory workflows. This comprehensive kit includes all reagents and materials necessary for rapid and efficient pistachio contamination monitoring throughout food processing and manufacturing operations.

Technical Specifications

Stock Code HU0030021
Test Principle Sandwich enzyme immunoassay (ELISA)
Format 96-well microplate
Total Incubation Time 60 minutes (20/20/20 minute intervals)
Calibration Standards 0, 1, 4, 10, 40 ppm
Detection Wavelength 450 nm (HRP-TMB chromogenic reaction)
Cross-Reactivity Profile Cashew 4%, Hazelnut 0.17%, Sunflower seed 0.0002%, Walnut 0.0008%, Pecan 0.0005%
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Food allergen screening in manufacturing facilities
  • Environmental surface monitoring in production areas
  • Finished product verification and release testing
  • Cross-contamination assessment in multi-allergen production environments
  • Regulatory compliance documentation (FSMA, HACCP, ALLERGEN LABELING)
  • Supply chain verification of pistachio-containing ingredients
  • Quality assurance in food processing operations

Frequently Asked Questions

What is the clinical significance of the 0.13 ppm LOD?
The limit of detection of 0.13 ppm enables identification of pistachio allergen at levels substantially below typical labeling thresholds, providing sensitive compliance screening and cross-contamination detection.
How does the sandwich immunoassay principle benefit pistachio detection?
The sandwich configuration uses capture and detection antibodies to form a specific immunocomplex, enhancing specificity and reducing false positives from cross-reactive allergens while maintaining high sensitivity.
What is the approximate time to results?
Total assay time is 60 minutes including three 20-minute incubation intervals with integrated wash steps, enabling same-shift reporting in most laboratory workflows.
Are results affected by food processing (heat, hydrolysis)?
The ELISA detects pistachio-derived epitopes; processed or hydrolyzed samples may show reduced reactivity. Validation with relevant matrices is recommended for specific applications.
How should cross-reactivity data be interpreted?
Reported cross-reactivity percentages indicate minimal interference from cashew, hazelnut, and other tree nuts under standard assay conditions, supporting specific pistachio allergen identification.
What sample preparation is required?
Refer to the comprehensive kit manual for validated extraction protocols specific to your food matrix type. Proper sample dilution and filtration optimize assay performance.