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EcoScript 5x First Strand cDNA Synthesis Kit

Fiyat Sorunuz

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Complete first strand cDNA synthesis kit from RNA templates containing all components in ready-to-use format. Suitable for RT-PCR and gene expression analysis. Used by adding only water and RNA sample.

Contains thermostable recombinant M-MuLV Reverse Transcriptase
Lower RNase H activity compared to AMV reverse transcriptase
RNase Inhibitor, Random Primers, and Oligo dT Primer provided ready-to-use
dNTP Mix and Reaction Buffer included in the kit
Only water and RNA template addition required for use
5x concentrated formulation

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Daha fazla detay

EcoScript 5x First Strand cDNA Synthesis Kit provides all components in a single solution for rapid and efficient first strand cDNA synthesis from RNA templates. It is a practical solution for laboratories conducting molecular biology and gene expression studies.

The kit utilizes recombinant thermostable M-MuLV Reverse Transcriptase enzyme, which demonstrates lower RNase H activity compared to AMV reverse transcriptase. This characteristic assists in obtaining more intact cDNA products even with long and structurally challenging RNA templates.

The kit contains thermostable M-MuLV Reverse Transcriptase, RNase Inhibitor, Random Primers, Oligo dT Primer, dNTP Mix, and Reaction Buffer ready-to-use. Users can set up the reaction by simply adding water and RNA template, which saves time and reduces pipetting errors.

Technical Specifications

Enzyme	Recombinant thermostable M-MuLV Reverse Transcriptase
RNase H Activity	Lower than AMV reverse transcriptase
Primer Content	Random Primers and Oligo dT Primer
Kit Contents	RT enzyme, RNase Inhibitor, primers, dNTP Mix, Reaction Buffer
Concentration	5x
User Addition Required	Water and RNA template