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ClearPeak 2x Probe Master Mix

Fiyat Sorunuz

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2× concentrated real-time fluorescent quantitative PCR pre-mix for probe-based detection

2× concentrated convenient pre-mix formulation
Double antibody-modified hot-start DNA polymerase
>95% polymerase activity inhibition at temperatures up to 55°C
Reduced non-specific amplification at low temperatures
Optimized buffer system for enhanced amplification efficiency
Simplified workflow — add templates, primers, and probes only
Faster PCR runtime compared to competitor formulations
Reduced electricity consumption for eco-friendly operation

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Daha fazla detay

ClearPeak 2x Probe Master Mix is a ready-to-use, 2× concentrated pre-mix formulation for real-time fluorescent quantitative PCR (qPCR) using the probe-based detection method. The master mix contains EcoTaq DNA Polymerase, optimized PCR buffer, dNTPs, Mg2+, enhancers, and stabilizers. Simply add templates, primers, and probes to initiate amplification. ClearPeak 2x Probe Master Mix features a double antibody-modified hot-start DNA polymerase with >95% polymerase activity inhibition at temperatures up to 55°C, effectively suppressing non-specific amplification at low temperatures. The unique PCR buffer system significantly enhances qPCR amplification efficiency and specificity.

Technical Specifications

Product Type	2× Probe Master Mix pre-mix
Concentration	2×
DNA Polymerase	EcoTaq DNA Polymerase (double antibody-modified hot-start)
Polymerase Activity Inhibition	>95% at temperatures up to 55°C
Key Components	EcoTaq DNA Polymerase, PCR buffer, dNTPs, Mg2+, enhancers, stabilizers
Application	Real-time fluorescent quantitative PCR (qPCR) with probes
Product Codes	PMM1, PMM5

Applications

Gene expression analysis
Copy number variation (CNV) detection
Pathogen detection and quantification
Quality control in molecular diagnostics
Research applications requiring high specificity and efficiency

Frequently Asked Questions

What components need to be added to the master mix?

You only need to add templates, primers, and probes to the ClearPeak 2x Probe Master Mix. The mix already contains all other necessary components for qPCR.

How does the hot-start polymerase reduce non-specific amplification?

The double antibody-modified hot-start polymerase is inhibited (>95%) at low temperatures, preventing non-specific amplification during reaction setup. Activity is restored at higher temperatures during PCR cycling.

What is the advantage of the optimized PCR buffer system?

The unique buffer formulation significantly enhances qPCR amplification efficiency and improves overall assay performance and signal reliability.

How does ClearPeak 2x Probe Master Mix reduce PCR runtime?

The optimized formulation enables faster amplification kinetics compared to competitor products, reducing total PCR time while maintaining high specificity and efficiency.