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ClearBand 20x Tris-Buffered Saline (TBS), pH 7.6 – TB2005

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20x TBS concentrate formulated for Western blot and ELISA washing steps. Prepared with ultrapure water, filter sterilized, and diluted to 1x working solution. Provides safe washing without disrupting antibody-antigen binding.

  • 20x concentrate formulation provides storage space efficiency
  • 1x working solution pH of 7.6±0.1
  • Prepared with ultrapure water
  • Filter sterilized
  • Washing without disrupting antibody-antigen binding
  • Suitable for Western blot and ELISA procedures

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ClearBand 20x Tris-Buffered Saline (TBS) is a pH-buffered salt solution supplied as a concentrate for use in Western blot and ELISA procedures. The concentrated formulation provides storage efficiency and is diluted to 1x working solution at the time of laboratory use.

The 1x working solution maintains a pH of 7.6±0.1, which preserves antibody-antigen binding interactions while enabling effective washing. This balance is critical in Western blot membrane washing and ELISA plate washing to prevent signal loss or background noise.

The product is prepared with ultrapure water and filter sterilized, reducing contamination risk and supporting experimental reproducibility. The concentrate formulation allows users to prepare 1x buffer in volumes tailored to specific experimental requirements.

It is a practical and reliable washing buffer solution for genetics and molecular biology laboratories conducting Western blot and ELISA assays.

Technical Specifications

Concentration 20x
Working pH (1x) 7.6±0.1
Preparation Ultrapure water
Sterilization Filter sterilization
Manufacturer ClearBand
SKU TB2005

Applications

  • Western blot membrane washing steps
  • ELISA plate washing steps
  • Antibody incubation pre- and post-wash buffer preparation

Frequently Asked Questions

How do I prepare 20x TBS for use?
Dilute the product with ultrapure water at a 1:20 ratio. The resulting 1x working solution will have a pH of 7.6±0.1.
Do I need to convert TBS to TBST?
This product does not contain Tween-20; for TBST commonly used in Western blot washing, Tween-20 must be added separately.
Is the product sterile?
Yes, it is filter sterilized and prepared with ultrapure water.