ChemoMetec Solution 15 (Hoechst 1ml) – NC-3000 Reagent

Solution 15 is a Hoechst-33342-based staining reagent compatible with the ChemoMetec NucleoCounter NC-3000 system. It stains DNA in both viable and non-viable cells, providing total cell enumeration and serving as a reference channel for various functional assays.

This solution functions as a counterstain in NC-3000 applications including FlexiCyte, Caspase Assay, Annexin V Assay, and GFP Transfection Efficiency testing. Hoechst-33342 is a membrane-permeable dye that generates signal in both live and dead cells, enabling determination of total cell population across all viability states.

A practical consumable for laboratory users performing cell culture, bioprocess monitoring, and apoptosis/viability analysis. Its formulation complies with NC-3000 protocols, requiring no additional optimization for routine use.

Technical Specifications

Applications

• Total cell enumeration with NC-3000
• Reference staining in FlexiCyte, Caspase, and Annexin V assays
• GFP Transfection Efficiency testing