BIO BASIC BS82314 EZ-10 Spin Column Plant RNA Miniprep Kit, 50prep

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BIO BASIC BS82314 EZ-10 Spin Column Plant RNA Miniprep Kit, 50 Preparations

Fiyat Sorunuz

Rapid spin column-based RNA extraction kit for plant tissue samples with polysaccharide and polyphenol removal

Effective polysaccharide and polyphenol removal from plant samples
Rapid spin column purification without ethanol gradient washing
Compatible with all plant tissue types and growth stages
Prevents polyphenol oxidation during extraction
RNA integrity preserved for molecular biology applications
50 preparations per kit
Room temperature storage (18-25°C)

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Daha fazla detay

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Daha fazla detay

The EZ-10 Spin Column Plant RNA Miniprep Kit is engineered for efficient total RNA isolation from diverse plant tissues. Plant samples contain high levels of polysaccharides and polyphenols that form insoluble complexes with RNA, complicating purification. This kit addresses these challenges through specialized lysis buffer formulation that effectively suppresses polyphenol oxidation and enables rapid polysaccharide precipitation and removal. The spin column-based workflow eliminates the need for ethanol gradient washes, streamlining RNA recovery while maintaining nucleic acid integrity. Purified total RNA is suitable for downstream applications including RT-PCR, Northern blot analysis, mRNA enrichment, RNase protection assays, and cell-free translation systems.

Technical Specifications

Catalog Number	BS82314
Kit Size	50 Preparations
Purification Method	Spin Column
DNase Treatment	No
Storage Temperature	18-25°C

Applications

Reverse transcription PCR (RT-PCR) from plant tissues
Northern blot analysis of plant gene expression
Poly(A)+ mRNA selection and enrichment
RNase protection assay (Northern blotting variant)
Plant transcriptomics and gene expression profiling
In vitro translation using plant-derived mRNA
Plant molecular biology research and diagnostics
Quality control RNA extraction from diverse plant species

Frequently Asked Questions

Why is polysaccharide removal critical for plant RNA extraction?

Plant cell walls contain high concentrations of polysaccharides that bind RNA and form insoluble aggregates. These complexes co-precipitate with RNA and contaminate the final product, interfering with downstream enzymatic reactions.

How does this kit prevent polyphenol oxidation?

The specialized lysis buffer contains antioxidant compounds and chelating agents that suppress polyphenol enzymatic oxidation, which would otherwise degrade RNA quality.

Is DNase treatment included in this kit?

No. This kit does not include DNase treatment. For DNA-free RNA, perform an optional on-column or solution DNase digestion after RNA elution.

What RNA quality can be expected?

Purified RNA is typically high-quality total RNA suitable for RT-PCR, Northern blotting, and transcriptome analysis. RNA integrity (RIN score) generally exceeds 7 when starting material is properly handled.

Can this kit be used for all plant species?

Yes, the kit is formulated for universal plant RNA extraction and is applicable to herbaceous plants, woody plants, seeds, and specialized tissues.