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ClearBand 10x Tris/Glycine/SDS Running Buffer (TGS05)

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Ready-to-use 10x Tris/Glycine/SDS running buffer for protein separation by SDS-PAGE. pH 8.3, filter sterilized, ready for dilution.

  • 10x concentrate, dilute before use
  • pH 8.3
  • Filter sterilized
  • Prepared with ultrapure water
  • Suitable for SDS-PAGE and Western blot applications
  • Consistent, reproducible electrophoresis results

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ClearBand 10x Tris/Glycine/SDS Running Buffer is a concentrated, ready-to-use buffer formulated for the separation of denatured proteins by SDS-PAGE electrophoresis. It delivers consistent and reproducible electrophoresis results for laboratories performing Western blotting and protein analysis.

The formulation is standardized at pH 8.3, ensuring predictable and reproducible protein migration within the gel matrix. The buffer is prepared with ultrapure water and filter sterilized during manufacturing, reducing the risk of particulate contamination and minimizing background noise in the electrophoresis system. Supplied at 10x concentration, it is diluted to 1x working concentration in the laboratory, providing practical benefits for storage and transport.

The Tris/Glycine/SDS buffer system is compatible with standard SDS-PAGE gels (e.g., Laemmli system) and delivers reliable results during the protein separation phase prior to Western blotting.

Technical Specifications

Manufacturer ClearBand
Product Code (SKU) TGS05
Concentration 10x
pH 8.3
Composition Tris/Glycine/SDS
Sterilization Filter sterilized
Preparation Prepared with ultrapure water
Working Concentration Dilute to 1x prior to use

Applications

  • Protein separation by SDS-PAGE
  • Electrophoresis step prior to Western blotting
  • Denatured protein analysis

Frequently Asked Questions

How is the buffer used?
The 10x concentrated buffer is diluted with ultrapure water to 1x working concentration before use.
Which gel systems is it compatible with?
It is compatible with standard Tris-Glycine based SDS-PAGE gels (e.g., Laemmli system).
Why is the pH value important?
The pH 8.3 value ensures consistent and predictable protein migration within the gel matrix.