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Transparent Band Bradford Reagent – Total Protein Assay Reagent

Fiyat Sorunuz

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Ready-to-use Bradford reagent for rapid total protein concentration measurement using Coomassie Brilliant Blue G-250

Ready-to-use formulation for rapid total protein quantitation
Contains Coomassie Brilliant Blue G-250 dye
Compatible with test tube and microplate format assays
Measurement wavelength: 595nm
Quick incubation protocol with simple mixing procedure
Requires standard curve generation per assay

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Daha fazla detay

Transparent Band Bradford Reagent is a ready-to-use total protein analysis reagent formulated for rapid measurement of total protein concentration. This reagent contains Coomassie Brilliant Blue G-250, which binds to basic and aromatic amino acids, providing a shift in absorption during protein quantitation. Transparent Band Bradford Reagent offers a simple-to-use test in test tube or microplate format: mix protein sample with assay reagent, incubate for a brief period, and measure absorbance at 595nm. Since the color response with Coomassie is non-linear with increasing protein concentration, a standard curve must be generated for each assay.

Technical Specifications

Measurement Wavelength	595nm
Format Compatibility	Test tube and microplate
Active Component	Coomassie Brilliant Blue G-250
Assay Type	Bradford Total Protein Assay

Applications

Total protein concentration quantitation in research samples
Protein concentration determination in biochemistry laboratories
Batch analysis of multiple protein samples
Quality control in protein purification workflows
Protein concentration verification in analytical applications

Frequently Asked Questions

What is the principle of Bradford protein assay?

The Bradford assay measures protein concentration based on the binding of Coomassie Brilliant Blue G-250 dye to basic and aromatic amino acids in proteins, resulting in an absorbance shift that can be measured at 595nm.

Why is a standard curve required?

The color response with Coomassie is non-linear with protein concentration; therefore, a standard curve must be generated for each assay to ensure accurate quantitation.

What formats can be used with this reagent?

Transparent Band Bradford Reagent is compatible with both test tube and microplate format assays.

What is the incubation requirement?

The reagent requires a brief incubation period after mixing the protein sample with the assay reagent before measuring absorbance.