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PBS Phosphate Buffered Saline (1x), pH 7.4 – PBS500

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Calcium and magnesium-free 1x PBS solution; a sterile buffered salt solution prepared for cell washing, dilution, and transport operations.

pH 7.4 balanced buffered salt solution
Calcium and magnesium-free formulation
Prepared with ultrapure water
Filter sterilized
Autoclaved
Ready-to-use 1x concentration

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Daha fazla detay

PBS500 is a ready-to-use 1x phosphate buffered saline solution for routine operations in cell culture laboratories. Balanced at pH 7.4, it maintains cells in an isotonic environment. The absence of calcium and magnesium ions prevents chelator interference during pre-trypsinization washing steps, making it the preferred choice for cell dissociation protocols.

The product is prepared with ultrapure water, subjected to filter sterilization and autoclaving to minimize contamination risk. It is suitable for pre-trypsinization cell washing, transport of tissue samples, cell counting dilutions, and preparation of various reagents.

Ideal for cell culture laboratories, tissue banks, and molecular biology applications seeking a reliable, standardized base solution.

Technical Specifications

Product Type	Phosphate Buffered Saline (PBS)
Concentration	1x
pH	7.4
Calcium/Magnesium Content	None
Preparation Water	Ultrapure water
Sterilization	Filter sterilization and autoclaving
SKU	PBS500

Applications

Pre-trypsinization cell washing
Transport of cell or tissue samples
Dilution for cell counting
Reagent preparation

Frequently Asked Questions

Why does PBS500 not contain calcium and magnesium?

It is formulated without calcium and magnesium ions to facilitate chelator removal from culture before trypsinization, preventing interference with cell dissociation protocols.

Is PBS500 sterile?

Yes, it is prepared with ultrapure water, subjected to filter sterilization, and autoclaved.

In which cell culture operations is PBS500 used?

It is used in routine cell culture operations including cell washing, sample/tissue transport, dilution, and reagent preparation.