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ClearPeak 2x SYBR Master Mix for qPCR

Fiyat Sorunuz

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High-performance 2x SYBR Master Mix for real-time fluorescent quantitative qPCR reactions with hot-start EcoTaq DNA Polymerase

Hot-start EcoTaq DNA Polymerase with antibody-based activation mechanism
Optimized qPCR buffer system for maximum amplification efficiency
Effective suppression of non-specific amplification products
Superior specificity and detection sensitivity
Compatible with rapid thermal cycling protocols
Wide quantitative dynamic range with excellent linearity
Fast reaction times—reduces runtime compared to competing formulations
Reduces overall PCR cycle time, lowering energy consumption

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Daha fazla detay

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Daha fazla detay

ClearPeak 2x SYBR Master Mix is a specialized pre-formulated reagent designed for real-time fluorescent quantitative qPCR reactions using the SYBR Green I dye method. The core component is EcoTaq DNA Polymerase, an antibody-based hot-start DNA polymerase that can be reactivated by heating at 95°C for 5 seconds. The formulation combines strong specificity and high detection sensitivity with an optimized buffer system specifically engineered for qPCR applications. The proprietary qPCR buffer system, when combined with hot-start enzyme technology, effectively suppresses non-specific product generation and significantly enhances amplification efficiency. Excellent for high-specificity and high-sensitivity qPCR reactions. Compatible with rapid qPCR cycling protocols. Delivers reliable standard curves enabling accurate, reproducible, and trustworthy quantification of target genes across a wide quantitative dynamic range.

Technical Specifications

Polymerase Type	EcoTaq DNA Polymerase (Hot-start, antibody-based)
Detection Method	SYBR Green I
Application	Real-time quantitative qPCR
Buffer System	Proprietary optimized buffer formulation
Quantitative Range	Wide dynamic range with excellent linearity

Applications

High-specificity gene quantification and expression analysis
Sensitive detection of target DNA sequences
Rapid qPCR assay development and validation
Gene copy number determination
Quality control and quality assurance testing
Research applications requiring precise quantitative results
High-throughput qPCR screening

Frequently Asked Questions

What is the primary advantage of ClearPeak 2x SYBR Master Mix?

The combination of hot-start enzyme technology with proprietary buffer optimization provides superior specificity, enhanced amplification efficiency, and significantly reduced non-specific product formation compared to standard formulations.

How does the hot-start mechanism work?

EcoTaq DNA Polymerase is antibody-based, keeping the enzyme inactive until heating to 95°C for 5 seconds, which denatures the blocking antibody and activates the polymerase, minimizing non-specific amplification during setup.

Is this product suitable for rapid qPCR protocols?

Yes, ClearPeak 2x SYBR Master Mix is optimized for rapid thermal cycling protocols, delivering faster reaction times while maintaining accuracy and specificity.

What are the environmental benefits?

Reduced PCR cycle times mean lower overall run duration, which translates to decreased energy consumption and a smaller environmental footprint per reaction.

What quantitative range can be achieved?

The formulation supports a wide quantitative dynamic range with excellent linearity and reproducibility, enabling reliable target gene quantification across multiple orders of magnitude.