AFG Scientific HEPES 100 gr – Cell Culture Buffer

HEPES is a non-toxic-to-cells hydrogen ion buffer widely used in cell culture and biochemical experiments. Manufactured by AFG Scientific at ≥99.5% purity, this product is suitable for laboratory applications requiring long-term and stable pH control.

HEPES is preferred in cell culture media, particularly over bicarbonate-based buffers, due to its lower susceptibility to CO2 fluctuations. It is typically used at 10-50 mmol/L concentration in culture medium solutions; usage around 20 mmol/L is known to provide adequate buffering capacity. It is a stable chemical in white powder form and can be stored at room temperature.

With molecular formula C8H18N2O4S and molecular weight 238.3 g/mol, HEPES serves as a reliable buffer component in cell culture, protein research, and general biochemistry laboratories.

Technical Specifications

Applications

• Cell culture medium buffering
• pH stabilization in biochemical experiments
• Protein purification and enzyme studies
• Molecular biology buffer solutions