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EcoPURE Genomic DNA Kit

Fiyat Sorunuz

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Rapid genomic DNA purification from diverse biological samples using chaotropic ions and silica membrane technology

Compatible with multiple sample types and biological sources
Chaotropic ion and silica membrane-based purification technology
Eliminates need for toxic phenol-chloroform extraction
No time-consuming alcohol precipitation steps
Rapid protocol: <25 minutes
High-quality genomic DNA yield
PCR, qPCR, sequencing, and enzyme-ready DNA
Cost-effective alternative to resin-based methods

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Daha fazla detay

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Daha fazla detay

EcoPURE Genomic DNA Kit is engineered for simple and efficient purification of high-quality genomic DNA from diverse sample types including whole blood, cultured cells, frozen or fresh tissues, rodent tails, yeast, gram-positive and gram-negative bacteria, insects, dried blood spots, and buccal swabs. The kit employs chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standardized protocol requires less than 25 minutes, and purified DNA can be used directly in PCR, qPCR, sequencing, and enzymatic reactions.

Technical Specifications

Protocol Time	<25 minutes
Sample Types	Whole blood, cultured cells, tissues, rodent tails, yeast, bacteria, insects, dried blood spots, buccal swabs
Purification Method	Chaotropic ions with silica-based membrane technology
DNA Applications	PCR, qPCR, sequencing, enzymatic reactions

Applications

Genomic DNA extraction from whole blood samples
DNA isolation from cultured cell lines
Tissue genomic DNA purification (fresh or frozen)
Bacterial DNA extraction (gram-positive and gram-negative)
Yeast and fungal DNA isolation
Insect genomic DNA preparation
Forensic DNA analysis from dried blood spots
Buccal swab sample processing
Rodent tail tissue DNA extraction
Preparation of samples for high-throughput sequencing
DNA template preparation for PCR and qPCR assays

Frequently Asked Questions

What makes EcoPURE different from traditional extraction methods?

EcoPURE eliminates the need for toxic phenol-chloroform extractions and expensive resin-based methods. Using chaotropic ions with silica membrane technology provides a safer, faster, and more cost-effective purification process without compromising DNA quality.

Can I use the purified DNA directly for downstream applications?

Yes. The purified DNA is of sufficient quality and purity for direct use in PCR, qPCR, DNA sequencing, and enzymatic reactions without additional treatment or dilution.

What is the typical protocol duration?

The standardized protocol requires less than 25 minutes from sample preparation to DNA elution, allowing for rapid processing of multiple samples.

Which sample types are compatible with this kit?

The kit is designed for comprehensive sample compatibility including whole blood, cultured cells, fresh or frozen tissues, rodent tails, yeast, gram-positive and gram-negative bacteria, insects, dried blood spots, and buccal swabs.

Is the purification process suitable for forensic applications?

Yes. The kit is effective for DNA extraction from challenging forensic samples, including aged dried blood spots, and provides DNA suitable for downstream forensic analysis and genotyping.