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Eco Spin Blood Total RNA Kit

Fiyat Sorunuz

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High-quality total RNA purification from whole blood including microRNAs using chaotropic ion and silica membrane technology

Purifies total RNA including microRNAs from whole blood samples
Processes up to 8 ml of starting material
Eliminates hazardous chemicals (phenol-chloroform, β-mercaptoethanol)
Rapid protocol: less than 40 minutes at room temperature
Compatible with downstream applications (cDNA synthesis, northern blotting, differential display)
Silica-based membrane technology for consistent yields

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Daha fazla detay

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Daha fazla detay

Eco Spin Blood Total RNA Kit is engineered for simple and efficient purification of high-quality total RNA, including small RNAs such as microRNAs, from up to 8 ml of whole blood. This kit eliminates the need for expensive resins, hazardous phenol-chloroform extraction, β-mercaptoethanol, or time-consuming alcohol precipitation by utilizing chaotropic ion and silica-based membrane technology. The standard protocol completes in less than 40 minutes at room temperature, and purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.

Technical Specifications

Starting Material	Up to 8 ml whole blood
RNA Type	Total RNA including microRNAs and small RNAs
Protocol Duration	Less than 40 minutes at room temperature
Technology	Chaotropic ion and silica membrane-based purification
Chemical Extraction	Phenol-chloroform free, β-mercaptoethanol free

Applications

cDNA synthesis and reverse transcription
Northern blotting and hybridization studies
Differential display analysis
Primer extension assays
mRNA selection and enrichment
Gene expression profiling
RNA quality assessment applications

Frequently Asked Questions

What is the maximum blood volume this kit can process?

The Eco Spin Blood Total RNA Kit is designed to process up to 8 ml of whole blood per purification.

Does the kit require hazardous chemicals like phenol-chloroform?

No. This kit uses chaotropic ion and silica membrane technology, eliminating the need for phenol-chloroform extraction, β-mercaptoethanol, or expensive resins.

How long is the standard purification protocol?

The complete protocol can be completed in less than 40 minutes at room temperature.

Can the purified RNA be used for microRNA studies?

Yes. The kit is specifically designed to preserve and purify small RNAs including microRNAs along with total RNA.

What downstream applications are compatible with this RNA?

Purified RNA is suitable for cDNA synthesis, northern blotting, differential display, primer extension, mRNA selection, and other standard molecular biology applications.