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EcoSpinLiquid Total RNA Kit – Liquid Sample Purification

Fiyat Sorunuz

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High-quality total RNA purification from 250 μl biological fluid samples using silica membrane technology

Simple and convenient one-tube purification workflow
Compatible with diverse biological fluid samples (saliva, serum, plasma, urine, etc.)
Eliminates expensive resins and hazardous reagents
No β-mercaptoethanol or lengthy alcohol precipitation required
Complete protocol in under 40 minutes at room temperature
High-purity RNA output for sensitive downstream applications
Cost-effective per-sample processing

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Daha fazla detay

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Daha fazla detay

EcoSpinLiquid Total RNA Kit is engineered for rapid and efficient purification of high-quality total RNA from 250 μl liquid samples including saliva, serum, plasma, urine, and other biological fluids. The kit employs chaotropic ion chemistry combined with silica-based membrane technology, eliminating the requirement for expensive resins, β-mercaptoethanol, or time-consuming alcohol precipitation steps. The standardized protocol completes in less than 40 minutes at room temperature, yielding RNA suitable for all routine downstream molecular applications.

Technical Specifications

Input Sample Volume	250 μl
Technology Platform	Silica-based membrane with chaotropic ions
Operating Temperature	Room temperature
Protocol Duration	<40 minutes
Sample Types	Saliva, serum, plasma, urine, biological fluids

Applications

Complementary DNA (cDNA) synthesis
Northern blotting analysis
Differential display PCR
Primer extension assays
mRNA enrichment and selection
Gene expression profiling
qRT-PCR applications

Frequently Asked Questions

What types of liquid samples are compatible with this kit?

EcoSpinLiquid Total RNA Kit is validated for saliva, serum, plasma, urine, and other biological fluids requiring 250 μl input volumes.

Is β-mercaptoethanol required for this protocol?

No, the kit eliminates the need for β-mercaptoethanol and other hazardous organic reagents through its proprietary chaotropic ion technology.

What is the total hands-on time for RNA purification?

The complete purification protocol requires less than 40 minutes at room temperature from sample input to purified RNA output.

Can purified RNA be directly used in downstream assays?

Yes, the RNA yield is suitable for cDNA synthesis, Northern blotting, differential display, primer extension, and mRNA selection without additional processing.

Does this method require expensive resins or columns?

No, the kit uses silica-based membrane technology, eliminating the need for costly resins and simplifying the purification workflow.