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Cell Cycle Analysis Kit (CCA20) – DNA Content Detection

Fiyat Sorunuz

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Flow cytometry-based kit for analyzing cell cycle phases (G0/G1, S, G2/M) by DNA content using PI fluorescent staining. Compatible with suspension and adherent cell cultures.

Cell cycle phase detection based on DNA content (G0/G1, S, G2/M)
PI (propidium iodide) fluorescent staining principle
Compatible with flow cytometry
Applicable to suspension and adherent cell cultures
Designed for cultured cell samples

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Daha fazla detay

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Daha fazla detay

The Cell Cycle Analysis Kit (CCA20) is designed to determine cell cycle phases (G0/G1, S, G2/M) in cultured cells by measuring DNA content. Fluorescent dyes such as PI (propidium iodide) bind to cellular DNA, and the fluorescence intensity measured by flow cytometry indicates which cell cycle phase the cell is in.

This method is widely used in cell proliferation, apoptosis, and cell cycle arrest studies; it provides a practical solution for researchers investigating drug effects, toxicity testing, or the impact of genetic manipulations on cell cycle progression. The kit is designed to work with both suspension and adherent cell cultures, enabling flexible application across different cell types.

Proper attention to staining duration and cell fixation steps according to the kit protocol is recommended for accurate and reproducible results.

Technical Specifications

Product Type	Cell Cycle Analysis Kit
SKU	CCA20
Detection Method	Fluorescent staining (PI) + Flow Cytometry
Sample Type Application	Suspension and adherent cell cultures
Staining Component	PI (propidium iodide)-based fluorescent dye
Category	Genetic Products / Cell Culture

Applications

Cell proliferation studies
Evaluation of cell cycle effects in drug and toxicity testing
Apoptosis and cell cycle arrest analysis
Investigation of genetic manipulation effects on cell cycle progression

Frequently Asked Questions

Which cell types can be used with this kit?

The kit is compatible with cultured suspension or adherent cells.

How are cell cycle phases detected?

PI fluorescent dye binds to DNA; the difference in fluorescence intensity measured by flow cytometry allows discrimination of cells in G0/G1, S, and G2/M phases.

What should be considered for result accuracy?

Working according to the kit protocol regarding staining duration, cell fixation, and dye concentration is important for reproducible results.