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10x PBS Phosphate Buffered Saline, pH 7.4 – PBS10x-05

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10x concentrated PBS solution free of calcium and magnesium. Prepared for cell culture washing, dilution, and Western blot applications, sterile filtered buffer.

  • 10x concentrated, dilute before use
  • pH 7.4, near physiological pH
  • Calcium and magnesium free
  • Prepared with ultrapure water
  • Sterile filtered and autoclaved
  • Suitable for cell culture and Western blotting applications

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10x PBS is one of the most frequently used buffers in cell culture and molecular biology laboratories. This product can be diluted to working concentration and used for cell washing, tissue sample transport, cell dilution for counting, and reagent preparation.

The product is free of calcium and magnesium; therefore, when used prior to trypsinization, it does not interfere with chelators and does not compromise cell dissociation protocols. It is prepared with ultrapure water, sterile filtered, and autoclaved, thereby minimizing contamination risk.

With a pH value of 7.4 close to physiological pH, it is suitable for cell washing and dilution procedures while maintaining cell viability. It can also be used in Western blotting applications for membrane washing and preparation of antibody dilution buffers. The 10x concentrated format provides users with dilution flexibility based on working volumes and saves storage space.

Technical Specifications

Concentration 10x
pH 7.4
Calcium/Magnesium Content Free
Sterilization Filter sterilization + autoclave
Preparation Water Ultrapure water
SKU PBS10x-05

Applications

  • Cell washing prior to trypsinization
  • Transport of cell or tissue samples
  • Dilution for cell counting
  • Reagent and buffer preparation
  • Western blot membrane washing and antibody dilution

Frequently Asked Questions

How should I dilute 10x PBS?
For 1x working concentration, add 9 volumes of ultrapure or distilled water to 1 volume of 10x PBS.
Does this PBS contain calcium and magnesium?
No, the product is free of calcium and magnesium, which is why it is preferred for washing prior to trypsinization.
Is the product sterile?
Yes, filter sterilization and autoclave procedures have been applied.