Trypan Blue %0.4 Solution – Cell Counting and Viability Testing Reagent (TRY-B100)

Trypan Blue %0.4 Solution is a ready-to-use reagent commonly utilized in cell culture laboratories for cell counting and viability determination. It offers a practical solution for researchers seeking to assess cell density before subculturing cells or proceeding to advanced in vitro/in vivo experiments.

This reagent operates on the dye exclusion principle: non-viable cells with compromised cell membranes take up the dye and stain blue, while healthy viable cells exclude the dye and remain colorless. This enables straightforward determination of viable/non-viable cell ratios using a hemocytometer or automated cell counter.

The %0.4 concentration is formulated to be compatible with standard cell counting protocols and is supplied ready for direct use. It is widely preferred in cell culture, drug development, and genetic research laboratories.

Technical Specifications

Applications

• Cell counting prior to cell culture subculturing procedures
• Determination of cell viability percentage
• Cell density control before in vitro/in vivo experiments