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VitaBright-43™ Solution – Live Cell Apoptosis Detection Reagent

Fiyat Sorunuz

Non-fluorescent probe for detecting early to mid-stage apoptosis via thiol-dependent fluorescence in living mammalian cells

  • Non-fluorescent probe with strong intracellular fluorescent product generation
  • Intensity-dependent staining reflects cellular redox status
  • Superior detection of early and mid-stage apoptosis in mammalian cells
  • Rapid and simple cell viability assessment
  • Detects changes in thiol levels (GSH) associated with apoptosis

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VitaBright-43™ is a non-fluorescent compound that generates robust fluorescent product within viable cells. The probe stains cells in an intensity-dependent manner that reflects cellular redox status. High fluorescence intensity indicates cells with reduced thiol levels. VitaBright-43™ serves as a superior marker for early and mid-stage apoptosis detection in mammalian cells. This solution enables users to detect changes in low thiol levels (such as GSH) in apoptotic cells or cells undergoing other pathological processes. Since low thiol levels are an indicator of overall cellular health status, this assay provides a fast and straightforward method for cell viability assessment.

Technical Specifications

Format Solution
Volume 100 µl
Active Component VitaBright-43™
Concentration 1 mM
Mass Concentration ~274 µg/mL
Percentage ~0.027%
Solvent DMSO
Compatible System NC-3000™

Applications

  • Detection of early and mid-stage apoptosis in mammalian cells
  • Assessment of cellular redox status
  • Measurement of intracellular thiol (GSH) level changes
  • Evaluation of cell viability and health status
  • Monitoring cells undergoing pathological processes
  • Flow cytometry applications
  • Fluorescence microscopy analysis

Frequently Asked Questions

Is VitaBright-43™ fluorescent in its native form?
No, VitaBright-43™ is non-fluorescent in solution. It becomes strongly fluorescent only when it enters viable cells and reacts with intracellular components.
What does fluorescence intensity indicate?
High fluorescence intensity indicates cells with elevated redox status and low thiol levels, which is characteristic of apoptotic or stressed cells.
Which cell types can be analyzed?
VitaBright-43™ is optimized for mammalian cells and can detect early to mid-stage apoptosis in various cell types.
How quickly can results be obtained?
VitaBright-43™ provides rapid detection, making it an efficient and straightforward method for cell viability assessment.
What is the relationship between thiol levels and cell health?
Low thiol levels (particularly GSH) are indicators of cellular stress, oxidative conditions, and apoptotic progression. They serve as biomarkers for overall cellular health status.
Is this product compatible with other systems?
Yes, Solution 19 – VitaBright-43™ is designed to be used in conjunction with NC-3000™ system.