VitaBright-48™ – Live Cell Apoptosis Detection Reagent

VitaBright-48™ is a non-fluorescent compound that generates potent fluorescent product within viable cells. It stains cells proportionally to their redox status, enabling intensity-dependent quantification. High fluorescence intensity indicates elevated intracellular thiol depletion. VitaBright-48™ serves as a biomarker for detection of mid to late-stage apoptosis in mammalian cell systems. The reagent enables researchers to detect dynamic alterations in low-molecular-weight thiol levels (such as GSH) in apoptotic cells and cells undergoing pathological processes. Since reduced thiol levels correlate with cellular health status, this assay provides a rapid, user-friendly approach for cell viability assessment.

Technical Specifications

Applications

• Detection of apoptotic cells in mammalian cell cultures
• Assessment of intracellular thiol depletion during programmed cell death
• Evaluation of cellular health status via redox state analysis
• Monitoring of pathological cellular processes
• Live-cell fluorescence microscopy and flow cytometry applications
• Cell viability screening in drug research