AFG Scientific Bromophenol Blue ACS Reagent, 5 g

Bromophenol Blue is a sulfonphthalein dye widely used in laboratories as a pH indicator and as a component of loading buffers in protein and nucleic acid electrophoresis. This ACS Reagent grade product delivers high purity for reliable and reproducible results in analytical applications.

Manufactured by AFG Scientific, this 5 gram package is identified by CAS No 115-39-9 and possesses the molecular formula C19H10Br4O5S with a molecular weight of 669.98 g/mol. It appears as a tan-orange colored powder and exhibits a melting point of 273°C. Room temperature storage capability facilitates routine laboratory use.

As a pH indicator, it exhibits a yellow-to-blue color transition in the approximate range of pH 3.0-4.6 (acidic-basic transition zone), making it preferred for titration studies and buffer systems. Additionally, it is widely employed in SDS-PAGE and agarose gel electrophoresis to provide visibility of sample loading buffers during sample introduction.

Technical Specifications

Applications

• pH indicator in titration studies
• Loading buffer component in protein and nucleic acid electrophoresis
• Color transition indicator in analytical chemistry laboratories