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Ethidium Bromide, Biotechnology Grade, 1G | BioShop ETB333

Fiyat Sorunuz

High-purity ethidium bromide for nucleic acid staining and fluorescence visualization in molecular biology research

  • Potent intercalating agent for double-stranded DNA intercalation
  • Bright red-orange fluorescence under UV excitation
  • High sensitivity for nucleic acid detection
  • Biotechnology-grade purity
  • Compatible with standard gel electrophoresis protocols
  • Suitable for gel documentation and imaging

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BioShop ETB333 Ethidium Bromide is a widely utilized molecular dye in biotechnology applications. It is the preferred reagent for fluorescent visualization of nucleic acids in DNA and RNA research. This potent intercalating agent binds to double-stranded DNA, emitting a bright red-orange fluorescent signal under UV excitation, offering exceptional sensitivity for genetic studies. Proper safety precautions must be observed during handling and use.

Technical Specifications

Molecular Formula C21H20BrN3
Molecular Weight 394.32 g/mol
CAS Number 1239-45-8
Package Size 1G
Storage Conditions Room temperature

Applications

  • Nucleic acid staining in genetic research
  • DNA/RNA gel electrophoresis
  • Fluorescence microscopy applications
  • Gel documentation and imaging
  • Molecular biology research

Frequently Asked Questions

What is the mechanism of ethidium bromide staining?
Ethidium bromide is an intercalating agent that inserts between DNA base pairs, forming a highly fluorescent complex. Upon UV excitation, it emits characteristic red-orange fluorescence.
What excitation and emission wavelengths are used?
Excitation occurs at 254-300 nm; emission maximum is approximately 590 nm in the red-orange region.
How should the product be stored?
Store at room temperature in a dark, dry location protected from light exposure and moisture to maintain stability.
What safety precautions are necessary?
Ethidium bromide is a known mutagen with potential carcinogenic properties. Use appropriate personal protective equipment, ensure adequate ventilation, and follow institutional biosafety protocols. Consult the Safety Data Sheet for comprehensive safety information.